|Rimler, Richard - Rick|
Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 10/6/1995
Publication Date: N/A
Citation: N/A Interpretive Summary:
Technical Abstract: Adhesion to host cells and internalization are significant features in the pathogenesis of many bacterial diseases. Pasteurella multocida circumvents those processes by production of a hyaluronic acid capsule. This research focused on the adhesive properties of P. multocida and demonstrated the role of hyaluronic acid in adherence to turkey air sac macrophages. Capsulated strain P. multocida (serotype A:3) and its non-capsulated variant (-:3) were examined in adherence assays. Only capsulated organisms adhered in large number to air sac macrophages. Capsule depolymerization with hyaluronidase increased phagocytosis, and addition of hyaluronic acid to macrophage cultures inhibited bacterial binding. Exposure of air sac macrophages to chondroitin sulfate B, an anionic polysaccharide similar to hyaluronic acid, did not affect adhesion and resistance to phagocytosis of capsulated bacteria. Macrophage surface oxidation with metaperiodate reduced bacterial adhesion, and trypsin treatment suppressed bacterial binding. Collectively, these data suggest that P. multocida adhesion to air sac macrophages is mediated by capsular hyaluronic acid through the recognition of a specific glycoprotein receptor expressed on the macrophages.