Submitted to: Poultry Science Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 8/16/1995
Publication Date: N/A
Technical Abstract: An alternative approach to mapping Type I marker genes was predicated on the frequent occurrence of single-base substitutions and indications that introns are less conserved than exons. Nucleotide sequence analysis of cloned polymerase chain reaction (PCR) products from the parents of the Jungle Fowl (JF) by White Leghorn (WL) East Lansing reference population, revealed single base substitutions in introns of several candidate (Type I marker) genes. A substitution in the intron of the JF allele of adenylate kinase 1 (AK1) generated a distinguishing BspHI cleavage site. Base substitutions in introns of aldolaseB (ALDOB), a lysosomal membrane protein gene (LAMP1) and vitellogenin 2 (VIT2) led to the design of allele-specific amplification of JF alleles using the PCR 3'-primer mismatch method. Segregation of 52 F2 backcross progeny revealed that AK1, ALDOB, LAMP1 and VIT2 were localized to linkage group E41, the Z chromosome, chromosome 1 and linkage group E43, respectively.