Author
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SUNDEN S L F - UNIV OF IOWA |
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KAPPES S M - 5438-01-30 |
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BUSINGA T - UNIV OF IOWA |
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STONE R T - 5438-01-30 |
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SHEFFIELD V C - UNIV OF IOWA |
|
Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only Publication Acceptance Date: 3/10/1995 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: In order to further delimit syntenic conservation between humans and cattle and to place additional genes on the bovine linkage map, we have mapped the bovine homologue of liver glycogen phosphorylase (PYGL) to bovine chromosome 7 (syntenic group U22). Using homology between human and rat muscle and live glycogen phosphorylase genes, a pair of primers was designed to flank an intron in both genes. Two sizes of product were observed when these primers were used to amplify bovine DNA; a 400 base pair product, which corresponds to the muscle glycogen phosphorylase gene (PYGM) in humans, and a 210 base pair product, which corresponds to the PYGL gene. Additional sequence analysis of human and rat intron and exon sequence will confirm the homology of the bovine products. Single stranded conformation polymorphism analysis of the bovine PCR products revealed a two allele polymorphism in the smaller product. This polymorphism was observed in 4 animals of Bos indicus breeding, but was not observed in 21 animals of straight Bos taurus breeding. The polymorphism was genotyped in the MARC back-cross reference families and subsequently mapped to chromosome 7 by linkage. The most likely location of the gene was between BM1853 and BM6117. PYGL is located on human chromosome 14. One other gene from this chromosome has been mapped to bovine chromosome 7; polymeric immunoglobulin receptor (PR). Mapping PYGL to bovine 7 extends the region of syntenic conservation and may indicate an additional breakpoint of synteny. |
