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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Dairy and Functional Foods Research » Research » Publications at this Location » Publication #408358

Research Project: In vitro Human Gut System: Interactions Between Diet, Food Processing, and Microbiota

Location: Dairy and Functional Foods Research

Title: Metagenomes and metagenome-assembled genomes from ex vivo fecal incubations of six unique donors

item Narrowe, Adrienne
item Liu, Linshu
item Scarino Lemons, Johanna
item Firrman, Jenni
item Mahalak, Karley
item DEYAERT, STEF - Cryptobiotix
item BAUDOT, AURELIEN - Cryptobiotix
item VAN DEN ABBEELE, PIETER - Cryptobiotix

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/18/2023
Publication Date: 1/18/2024
Citation: Narrowe, A.B., Liu, L.S., Scarino Lemons, J.M., Firrman, J., Mahalak, K.K., Deyaert, S., Baudot, A., Van Den Abbeele, P. 2024. Metagenomes and metagenome-assembled genomes from ex vivo fecal incubations of six unique donors. Microbiology Resource Announcements.

Interpretive Summary: The human gut microbiome is an important component of human health. Studies of the human gut microbiome work to identify specific bacteria or groups of bacteria associated with improved health, however the microbiomes of different individuals can vary in composition, making it difficult to identify universal characteristics. Additionally, microorgansisms that seem to be very similar can also contain different functional capacities. By sequencing and reconstructing microbial genomes from multiple individuals, we can work toward a more broad characterization of the potential found within the human gut microbiome. Large-scale data sharing facilitates this type of research and the dataset presented by this study adds to the catalog of human gut microbiome data.

Technical Abstract: The gut microbiome is critical to human health and can both transform and be transformed by food components and bioactive products. Yet inter-individual differences in gut microbiome composition can affect the metabolism of these compounds. We used incubations of fecal samples from six adult donors to assess the effects of selected plant extracts on the human gut microbiome. From this, we present a donor-specific collection of 78 metagenomes (13/donor) and 143 metagenome-assembled genomes (MAGs) representing the gut microbiomes of six healthy adult human donors. In addition to adding to the catalog of publicly available human gut MAGs, this resource permits a genome-resolved look into microbial co-occurrence across six individuals.