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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Dairy and Functional Foods Research » People & Locations » Karley Mahalak
Karley K Mahalak
Dairy and Functional Foods Research
Research Molecular Biologist

Phone: (215) 836-6922
Fax:

USDA,REE,ARS,NAA,ERRC
600 E MERMAID LANE
WYNDMOOR, PA 190388598

(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)

Projects
In vitro Human Gut System: Interactions Between Diet, Food Processing, and Microbiota
In-House Appropriated (D)
  Accession Number: 439225
Fatty Acids Metabolism in the Gastrointestinal Tract Using In Vitro Enteroid Models Derived from Mice and Humans
Non-Assistance Cooperative Agreement (S)
  Accession Number: 437564

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Lacticaseibacillus rhamnosus strain GG (LGG) regulate gut microbial metabolites, an in vitro study using three mature human gut microbial cultures in a simulator of human intestinal microbial ecosystem (SHIME) Reprint Icon - (Peer Reviewed Journal)
Liu, L.S., Narrowe, A.B., Firrman, J., Mahalak, K.K., Bobokalonov, J., Scarino Lemons, J.M., Bittinger, K., Daniel, S., Tanes, C., Mattei, L., Friendman, E., Soares, J.W., Masuko, K., Zeng, W., Tomasula, M.M. 2023. Lacticaseibacillus rhamnosus strain GG (LGG) regulate gut microbial metabolites, an in vitro study using three mature human gut microbial cultures in a simulator of human intestinal microbial ecosystem (SHIME). Foods. https://doi.org/10.3390/foods12112105.
Fructooligosaccharides (FOS) differentially modifies the in vitro gut microbiota in an age-dependent manner. Reprint Icon - (Peer Reviewed Journal)
Mahalak, K.K., Firrman, J., Narrowe, A.B., Hu, W., Bittinger, K., Moustafa, A., Liu, L.S. 2023. Fructooligosaccharides (FOS) differentially modifies the in vitro gut microbiota in an age-dependent manner. Frontiers in Nutrition. https://doi.org/10.3389/fnut.2022.1058910.
Persistence of the probiotic Lacticaseibacillus rhamnosus strain GG (LGG) in an in vitro model of the gut microbiome Reprint Icon - (Peer Reviewed Journal)
Mahalak, K.K., Firrman, J., Bobokalonov, J., Narrowe, A.B., Bittinger, K., Daniel, S., Tanes, C., Mattei, L., Zeng, W., Soares, J.W., Kobori, M., Scarino Lemons, J.M., Tomasula, M.M., Liu, L.S. 2022. Persistence of the probiotic Lacticaseibacillus rhamnosus strain GG (LGG) in an in vitro model of the gut microbiome. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms232112973.
Changes to environmental pH alters the colon microbial community in terms of both structure and function: An in vitro analysis Reprint Icon - (Peer Reviewed Journal)
Firrman, J., Liu, L.S., Mahalak, K.K., Tu, V., Tanes, C., Bittinger, K., Bobokalonov, J., Mattei, L., Zhang, H., Van Den Abeele, P. 2022. Changes to environmental pH alters the colon microbial community in terms of both structure and function: An in vitro analysis. Applied and Experimental Microbiology. https://academic.oup.com/femsec/article/98/5/fiac038/6564177.
Analysis of the ability of capsaicin to modulate the human gut microbiota in vitro Reprint Icon - (Peer Reviewed Journal)
Mahalak, K.K., Bobokalonov, J., Firrman, J., Williams, R., Evans, B., Fanelli, B., Soares, J., Liu, L.S., Kobori, M. 2022. Analysis of the ability of capsaicin to modulate the human gut microbiota in vitro. Nutrients. https://doi.org/10.3390/nu14061283.
Changes to environmental pH alters the colon microbial community in terms of both structure and function: An in vitro analysis - (Abstract Only)
Environmental pH is a determinant of the gut microbiota community composition and production of short chain fatty acids - (Abstract Only)
Comparative analysis of the gut microbiota cultured in vitro using a single colon versus a 3-stage colon experimental design Reprint Icon - (Peer Reviewed Journal)
Firrman, J., Liu, L.S., Mahalak, K.K., Tanes, C., Bittinger, K., Bobokalonov, J., Van Den Abbeele, P., Mattei, L., Zhang, H. 2021. Comparative analysis of the gut microbiota cultured in vitro using a single colon versus a 3-stage colon experimental design. Applied Microbiology and Biotechnology. volume 105, pages 3353–3367. https://link.springer.com/article/10.1007/s00253-021-11241-x.
Triclosan has a robust, yet reversible impact on human gut microbial composition in vitro. Reprint Icon - (Peer Reviewed Journal)
Mahalak, K.K., Firrman, J., Lee, J., Bittinger, K., Nunez, A., Bobokalonov, J., Arango-Argoty, G., Zhang, L., Zhang, G., Liu, L.S. 2020. Triclosan has a robust, yet reversible impact on human gut microbial composition in vitro. PLoS One. 15(6): 1-22. https://doi.org/10.1371/journal.pone.0234046.
Impact of steviol glycosides and erythritol on the human and Cebus apella gut microbiome Reprint Icon - (Peer Reviewed Journal)
Mahalak, K.K., Firrman, J., Tomasula, M.M., Nunez, A., Lee, J., Bittinger, K., Rinaldi, W., Liu, L.S. 2019. Impact of steviol glycosides and erythritol on the human and Cebus apella gut microbiome. Journal of Agricultural and Food Chemistry. Pages A-I. https://doi.org/10.1021/acs.jafc.9b06181.
Metagenomic assessment of the Cebus Apella gut microbiota Reprint Icon - (Peer Reviewed Journal)
Firrman, J., Tanes, C., Bittinger, K., Mahalak, K.K., Rinaldi, W., Liu, L.S. 2019. Metagenomic assessment of the Cebus Apella gut microbiota. American Journal of Primatology. https://doi.org/10.1002/ajp.23023.