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ARS Home » Research » Publications at this Location » Publication #31992
Title: ANALYSES OF MAB REACTIVE WITH PORCINE CD8

Author
item SAALMULLER A
item AASTED B
item CANALS A
item DOMINGUEZ J
item GOLDMAN THERESA
item Lunney, Joan
item MAUER S
item PESCOVITZ M D
item POSPISIL R
item SALMON H

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/1/1994
Publication Date: N/A
Citation: N/A

Interpretive Summary: Based on FCM analyses 6 mAb: 76-2-11 (#004), PT36B (#051), PT81B (#052), PG93A (#053), 11/295/33 (#108) and 11/122/28 (#109) recognizing the porcine CD8 antigen were identified to cluster together in the T5 cluster. Epitope analyses using biotinylated mAb 11/295/33 (#108) and 76-2-11 (#004) led to the determination of two epitopes CD8a and CD8b, the first recognized by mAb 76-2-11 (#004), PT36B (#051) and PT81B (#052); second by mAb PG93A (#053), 11/295/33 (#108) and 11/122/28 (#109). Because of missing data about the molecular mass of the antigen recognized by mAb PT36B (#051), PT81B (#052) and PG93A (#053), these mAb received the prefix "w" for their respective subcluster.

Technical Abstract: Based on FCM analyses 6 mAb: 76-2-11 (#004), PT36B (#051), PT81B (#052), PG93A (#053), 11/295/33 (#108) and 11/122/28 (#109) recognizing the porcine CD8 antigen were identified to cluster together in the T5 cluster. Epitope analyses using biotinylated mAb 11/295/33 (#108) and 76-2-11 (#004) led to the determination of two epitopes CD8a and CD8b, the first recognized by mAb 76-2-11 (#004), PT36B (#051) and PT81B (#052); second by mAb PG93A (#053), 11/295/33 (#108) and 11/122/28 (#109). Because of missing data about the molecular mass of the antigen recognized by mAb PT36B (#051), PT81B (#052) and PG93A (#053), these mAb received the prefix "w" for their respective subcluster.