ANALYSES OF MAB REACTIVE WITH PORCINE CD8

Authors: SAALMULLER A - TUBINGEN, GERMANY; AASTED B - ROYAL VET UNIV, DENMARK; CANALS A - INIA, MADRID SPAIN; DOMINGUEZ J - INIA, MADRID, SPAIN; GOLDMAN THERESA - 1265-20-00; Lunney, Joan; MAUER S - INIA, MADRID, SPAIN; PESCOVITZ M D - IN UNIV, INDIANA; POSPISIL R - PRAGUE, CZECH REPUBLIC; SALMON H - INRA, MONNAIE, FRANCE

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/1/1994
Publication Date: N/A
Citation: N/A

Interpretive Summary: Based on FCM analyses 6 mAb: 76-2-11 (#004), PT36B (#051), PT81B (#052), PG93A (#053), 11/295/33 (#108) and 11/122/28 (#109) recognizing the porcine CD8 antigen were identified to cluster together in the T5 cluster. Epitope analyses using biotinylated mAb 11/295/33 (#108) and 76-2-11 (#004) led to the determination of two epitopes CD8a and CD8b, the first recognized by mAb 76-2-11 (#004), PT36B (#051) and PT81B (#052); second by mAb PG93A (#053), 11/295/33 (#108) and 11/122/28 (#109). Because of missing data about the molecular mass of the antigen recognized by mAb PT36B (#051), PT81B (#052) and PG93A (#053), these mAb received the prefix "w" for their respective subcluster.

Technical Abstract: Based on FCM analyses 6 mAb: 76-2-11 (#004), PT36B (#051), PT81B (#052), PG93A (#053), 11/295/33 (#108) and 11/122/28 (#109) recognizing the porcine CD8 antigen were identified to cluster together in the T5 cluster. Epitope analyses using biotinylated mAb 11/295/33 (#108) and 76-2-11 (#004) led to the determination of two epitopes CD8a and CD8b, the first recognized by mAb 76-2-11 (#004), PT36B (#051) and PT81B (#052); second by mAb PG93A (#053), 11/295/33 (#108) and 11/122/28 (#109). Because of missing data about the molecular mass of the antigen recognized by mAb PT36B (#051), PT81B (#052) and PG93A (#053), these mAb received the prefix "w" for their respective subcluster.