Location: Molecular Plant Pathology LaboratoryTitle: GUS reporter gene expression from Beta vulgaris root-specific promoters Author
Submitted to: Annual Beet Sugar Development Foundation Research Report
Publication Type: Research Technical Update
Publication Acceptance Date: 3/29/2013
Publication Date: 4/12/2013
Citation: Smigocki, A.C., Padmanaban, S., Braza, R. 2013. GUS reporter gene expression from Beta vulgaris root-specific promoters. Annual Beet Sugar Development Foundation Research Report. Available: http://www.bsdf-assbt.org/assbt/assbt.htm. Interpretive Summary:
Technical Abstract: To develop transgenic sugar beet with specialized agronomic traits for insect and disease tolerance and enhanced sugar accumulation and storage, a larger arsenal of constitutive, tissue-specific and temporal promoters is required. In the present study, a series of sugar beet promoters were tested for root specific expression. Fusion of the promoters to the GUS reporter gene was used to determine promoter driven expression patterns in transgenic sugar beet hairy roots used as an in vitro model for roots. Of the three sugar beet promoters that were analyzed, AR29 appears to be the strongest and also wound inducible in the hairy root lines. Two other promoters, AR25 and AR27, exhibited lower level of root expression that was similar to a control constitutive promoter CaMV 35S (AR31). However, unlike the 35S promoter, the AR25, AR27 and AR29 promoters were all wound-inducible thus making them more attractive for driving the expression of resistance genes in plants attacked by root pests and pathogens.