|Sun, Jianghoa - JOHNS HOPKINS UNIVERSITY|
|Liu, Xianjin - JIANGSU ACADEMY AGRICULTURAL SCIENCES|
|Yang, Tianbao - U.S. DEPARTMENT OF AGRICULTURE (USDA)|
|Solvin, Janet - U.S. DEPARTMENT OF AGRICULTURE (USDA)|
Submitted to: Analytical Biochemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/11/2013
Publication Date: 2/11/2013
Citation: Sun, J., Liu, X., Yang, T., Solvin, J., Chen, P. 2013. Profiling polyphenols of two diploid strawberry (Fragaria vesca) inbred lines using UHPLC-HRMSn. Analytical Biochemistry. 5:2945-53.
Interpretive Summary: The regular strawberry is octoploid (organism that has eight complete sets of chromosomes). This study analyzed phenolic compounds in the fruits of two diploid (organism that has two complete sets of chromosomes) strawberries inbred lines (Ruegen and YW5AF7). The study found anthocyanin composition of the fruit changes during ripening stages and between the two breeds of strawberries. Compared to the regular strawberry, several new phenolic compounds were found. For instance, peonidin 3-O-malonylglucoside is unique for Ruegen fruit and taxifolin 3-O-arabinoside is reported in Ruegen and YW5AF7 fruits for the first time. These results provide the basis for comparative analysis of polyphenolic compounds in yellow and red diploid strawberries, as well as diploid and cultivated octoploid strawberries.
Technical Abstract: Phenolic compounds in the fruits of two diploid strawberries (Fragaria vesca f. semperflorens) inbred lines-Ruegen F7-4 (a red fruited genotype) and YW5AF7 (a yellow fruited genotype) were characterized using ultra high-performance liquid chromatography in tandem with high resolution mass spectrometry (UHPLC-HRMSn). The changes of anthocyanin composition during fruit development and between Ruegen F7-4 and YW5AF7 were studied. About 74 phenolic compounds, including taxifolin 3-O-arabinoside, glycosides of quercetin, kaempferol, cyanidin, pelargonidin, peonidin, methyl ellagic acid and ellagic acid, flavonols, and ellagitannins were characterized. Compared to the commercial octoploid strawberry, unique phenolic compounds were found in F. vesca fruits, such as taxifolin 3-O-arabinoside (both) and peonidin 3-O-malonylglucoside (Ruegen F7-4). The results provide the basis for comparative analysis of polyphenolic compounds in yellow and red diploid strawberries, as well as with the commercial octoploid strawberries.