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United States Department of Agriculture

Agricultural Research Service

Research Project: Managing Diseases and Pests of Honey Bees to Improve Queen and Colony Health

Location: Bee Research Laboratory

Title: Standard methodologies for Nosema apis and N. ceranae research

item Ingemar, Fries
item Chauzat, Marie-pierre
item Chen, Yanping - Judy
item Doublet, Vincent
item Genersch, Elke
item Gisder, Sebastian
item Higes, Mariano
item Mcmahon, Dino
item Martin-hernandez, Raquel
item Natsopoulou, M
item Paxton, Robert
item Tanner, Gina
item Webster, Thomas
item Williams, Geoffrey

Submitted to: The COLOSS BEEBOOK- standard methodologies in Apis mellifera research
Publication Type: Book / Chapter
Publication Acceptance Date: 6/14/2012
Publication Date: 10/29/2012
Citation: Ingemar, F., Chauzat, M., Chen, Y., Doublet, V., Genersch, E., Gisder, S., Higes, M., Mcmahon, D.P., Martin-Hernandez, R., Natsopoulou, M.E., Paxton, R.J., Tanner, G., Webster, T.C., Williams, G.R. 2012. Standard methodologies for Nosema apis and N. ceranae research. Journal of Apicultural Research. 52(1):1-28.

Interpretive Summary:

Technical Abstract: The international research network COLOSS (Prevention of honey bee COlony LOSSes) was established to coordinate efforts towards improving the health of western honey bee at the global level. The COLOSS BEEBOOK contains a collection of chapters intended to standardized methodologies for monitoring and research on honey bee colony losses. This Nosema chapter covers methods used for working on species of Nosema apis and N. ceranae in the field and in the laboratory. The field methods section concerns colony level infections and includes suggestions for standardized procedures regarding sampling, field trials and infection control studies. The lab method section concerns both colony level and individual bee infections and includes procedures for describing infection levels, Nosema spp. identification, standardization of cage trials, inoculation methods, incubation conditions and in vitro rearing of Nosema spp. Suggested procedures for different types of experiments are also included. The chapter should be a valuable resource for students, scientists, apiary inspectors who work on honey bees.

Last Modified: 10/17/2017
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