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Title: Capillary electrophoresis to quantitate gossypol enantiomers in cottonseed and flower petals

item VSHIVKOV, SERGEY - Institute Of Bioorganic Chemistry
item PSHENICHNOV, EGOR - Institute Of Bioorganic Chemistry
item GOLUBENKO, ZAMIRA - Bioorganic Chemistry Institute
item AKHUNOV, ALIK - Bioorganic Chemistry Institute
item NAMAZOV, SHADMAN - Uzbekistan Cotton Research Institute
item Stipanovic, Robert - Bob

Submitted to: Journal of Chromatography B
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/22/2012
Publication Date: 10/10/2012
Citation: Vshivkov, S., Pshenichnov, E., Golubenko, Z., Akhunov, A., Namazov, S., Stipanovic, R.D. 2012. Capillary electrophoresis to quantitate gossypol enantiomers in cottonseed and flower petals. Journal of Chromatography B. 908:94-97.

Interpretive Summary: Gossypol is a chemical that occurs naturally in various tissues in the cotton plant including cottonseed. Gossypol helps protect the plant form insects that consume plant foliage; since gossypol is toxic to non-ruminant animals, its occurrence in cottonseed meal can be a concern when it is used in animal feed. Thus, it is important to determine the quantity of gossypol in various plant tissues. We have developed a new method to detect gossypol in plant tissues that can detect the compound at very low concentrations and is faster and more economical to run than currently published methods.

Technical Abstract: Gossypol occurs as a mixture of enantiomers in cotton plant tissues including seed and flower petals. The (-)-enantiomer is less toxic to non-ruminant animals. Efforts to breed cottonseed with a low percentage of (-)-gossypol requires the determination of the (+)- to (-)-gossypol ratio in seed and flower petals. We report a method to quantitatively determine the total gossypol and percent of its enantiomers in tissues from cotton using high performance capillary electrophoresis (HPCE). The method utilizes a borate buffer at pH 9.3 using a capillary with internal diameter of 50 micron, effective length of 24.5 cm, 15 kV and cassette temperature of 15°C. This method provides high accuracy and reproducible results with a limit of detection of the individual enantiomers of less than 36ng/mL providing base line separation in less than 6 minutes.