|De Leon, Jesus|
Submitted to: Meeting Abstract
Publication Type: Other
Publication Acceptance Date: 2/28/2012
Publication Date: N/A
Citation: N/A Interpretive Summary:
Technical Abstract: Phyllocoptruta olevoria (Acari: Eriophyidae) or the citrus rust mite (CRM) is one of the most economically important mite pests of citrus worldwide. CRM originated from Asia, however it is found in most citrus producing areas, such as, Brazil, Morocco and the United States. Though CRM is a major pest and studied worldwide, there are currently no molecular studies available. Molecular studies are important because they can provide a baseline of knowledge that can be applied to management programs, such as, proper taxonomic identification and point of origin. In this study we discuss DNA extraction techniques, optimization of mitochondrial gene, cytochrome oxidase subunit I (COI) and a nuclear ribosomal gene, internal transcribed spacer region I (ITSI), for molecular analysis of CRM. We also implemented inter-simple sequence repeat polymerase chain reactions (ISSR-PCR), which has been proven to be a valuable tool for population genetic studies because they can assist in determining genetic structure and resolving genetic relationships.