Submitted to: Current Opinion in Microbiology
Publication Type: Review Article
Publication Acceptance Date: 7/25/2011
Publication Date: 8/10/2011
Citation: Filiatrault, M.J. 2011. Progress in prokaryotic transcriptomics. Current Opinion in Microbiology. 14(5):579-586.
Technical Abstract: Genome-wide expression studies transformed the field of transcriptomics and made it feasible to study global gene expression in extraordinary detail. These studies have revealed an enhanced view of the transcriptional landscape and have yielded many biological insights. Particularly, we are now realizing that transcriptomes of prokaryotes are much more complex than once thought. Recent advances in microbial transcriptome analyses are highlighted in this review. Areas of progress include, the development of molecular techniques optimized for studying complete prokaryotic transcriptomes that minimize the abundance of ribosomal RNAs in RNA samples as well as the development of novel methods to create transcriptome libraries. Technological advances have led to a new emphasis in areas such as in vivo gene expression studies and metatranscriptomics.