|WAKEFIELD, LAURA - Cornell University - New York|
|GADOURY, DAVID - Cornell University - New York|
|SEEM, BOB - Cornell University - New York|
Submitted to: International Workshop on Grapevine Downy and Powdery Mildew Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 9/20/2010
Publication Date: 11/1/2010
Citation: Wakefield, L., Cadle Davidson, L.E., Gadoury, D., Seem, B. 2010. Sporulation in Erysiphe necator: signals, differential gene expression and possible implications for disease management. International Workshop on Grapevine Downy and Powdery Mildew Proceedings. 212-213.
Technical Abstract: Abundant production of conidia is a driving factor for epidemics of grape powdery mildew (Erysiphe necator (syn. Uncinula necator). Previous investigations revealed evidence for a signal that coordinates the onset of asexual reproduction. The genetic basis for this signal in powdery mildews had not been previously described. The goal of this research was to identify sequences differentially expressed during vegetative growth and throughout asexual and sexual reproduction in E. necator. We used cDNA-AFLP analysis to identify sequences associated with the developmental stages. Our investigations show some overlap between sporulation associated sequences in E. necator and model systems, but also indicate the fungus may substantially rely on unique genes.