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ARS Home » Northeast Area » Geneva, New York » Grape Genetics Research Unit (GGRU) » Research » Publications at this Location » Publication #253145

Title: Developmnent of molecular markers for powdery mildew resistance in grapevines

item MAHANIL, SIRAPRAPA - Foreign Agricultural Service (FAS, USDA)
item Garris, Amanda
item Owens, Christopher
item Ramming, David
item Cadle-Davidson, Lance

Submitted to: Acta Horticulturae
Publication Type: Proceedings
Publication Acceptance Date: 9/15/2010
Publication Date: 7/20/2014
Citation: Mahanil, S., Garris, A.J., Owens, C.L., Ramming, D.W., Cadle Davidson, L.E. 2014. Developmnent of molecular markers for powdery mildew resistance in grapevines. Acta Horticulturae. 1046:91-99.

Interpretive Summary:

Technical Abstract: Molecular markers have been used as a tool for plant breeding for two decades. Markers linked to disease resistance can be used to pyramid resistance genes for improved durability and to improve the efficiency of evaluation. To develop molecular markers linked to resistance to grape powdery mildew (Erysiphe necator), Vitis romanetti, a source of resistance from China, was used to create a mapping population. A pseudobackcross 2 population was produced by crossing C87-41 ((V. romanetti x V. vinifera) x V. vinifera) with B70-57 (V. vinifera). Resistance was assessed in multiple locations and years by both natural and artificial inoculation. A genetic map was constructed using one CAP, 127 SSRs and 27 SNPlex markers. This source of disease resistance is qualitative, fits a single-resistance gene model and was mapped to linkage group 18. A screen of eighteen offspring with a 9,000 SNP Infinium genotyping microarray identified 20 SNPs (across 3.4 Mb) on linkage group 18 that are tightly linked with the resistance locus. Together these markers will be a valuable tool for marker assisted selection of powdery mildew resistance (from V. romanetti).