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Title: Gene Expression patterns in cryogenically stored Arabidopsis thaliana shoot tips

Author
item BASU, CHHANDAK - University Of Northern Colorado
item Volk, Gayle
item Henk, Adam

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/30/2007
Publication Date: 8/2/2007
Citation: Basu, C., Volk, G.M., Henk, A.D. 2007. Gene Expression patterns in cryogenically stored Arabidopsis thaliana shoot tips. Meeting Abstract for the 5th Canadian Plant Genomics Workshop. July 30,-August 2, 2007, Vancouver, BC, Canada pp. 56.

Interpretive Summary: The genes expressed in response to cryostress in plant shoot tips are not known. In this project we compared the gene expression patterns in untreated, cryoprotectant-treated, and recovering shoot tips using differential display methods. This project identified two genes that appeared to be differentially expressed. The first is a calcium ion binding protein responsive to desiccation in Arabidopsis and the second is an osmotically responsive gene, Real-time PCR will be used to confirm results. These initial results indicate that Arabidopsis can serve as a model system for the investigation of cryopreservation gene expression patterns.

Technical Abstract: The genes expressed in response to cryostress in plant shoot tips are not known. In this project we compared the gene expression patterns in untreated, cryoprotectant-treated, and recovering shoot tips using differential display methods. This project identified two genes that appeared to be differentially expressed. The first is a calcium ion binding protein responsive to desiccation in Arabidopsis and the second is an osmotically responsive gene, Real-time PCR will be used to confirm results. These initial results indicate that Arabidopsis can serve as a model system for the investigation of cryopreservation gene expression patterns.