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Title: Coordination ability of insect kinin analogs

item LODYGA-CHRUSCHINSKA, E - Technical University Of Lodz
item OLDZIEJ, S - Technical University Of Lodz
item SANNA, D - National Research Council - Italy
item MICERA, G - University Of Sassari
item CHRUSCINSKI, L - Technical University Of Lodz
item KACZMAREK, KRISZTOF - Technical University Of Lodz
item Nachman, Ronald
item ZABROCKI, JANUSZ - Technical University Of Lodz
item SYKULA, A - Technical University Of Lodz

Submitted to: Polyhedron
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/25/2008
Publication Date: 2/15/2009
Citation: Lodyga-Chruschinska, E., Oldziej, S., Sanna, D., Micera, G., Chruscinski, L., Kaczmarek, K., Nachman, R.J., Zabrocki, J., Sykula, A. 2009. Coordination ability of insect kinin analogs. Polyhedron. 28:485-492.

Interpretive Summary: Because of problems with the development of resistance to conventional pesticides, there is a critical need for new concepts and alternative approaches in controlling insect pests. The basic premise of this research is that neuropeptides (short chains of amino acids) serve as potent messengers in insects to regulate vital functions. Nevertheless, neuropeptides in and of themselves hold little promise as pest control agents because of susceptibility to being degraded in the target insect. We must design neuropeptide mimics that resist degradation by enzymes in the digestive tract and blood of pest insects and interact with the active site within the agricultural or medical pest in such a way as to either over-activate or block critical, neuropeptide-regulated life functions. We report on an investigation into the role of dissolved copper in the specific shapes that a mimic of the insect kinin neuropeptides adopt to interact and activate their respective active sites within the target pests. This discovery will aid in the design of neuropeptide-like compounds capable of disrupting critical life functions in insects. This work brings us one step closer to the development of practical neuropeptide-like substances that will be effective in controlling insect pests in an environmentally friendly fashion.

Technical Abstract: Spectroscopic data, including electronic absorption, CD and EPR results, as well as theoretical calculations have shown that the insertion of 4-aminopyroglutamate, a novel cis-ppetide bond mimic, in the insect kinin peptide leads to an effective ligand towards Cu(II) ions at basic pH ranges. The 4-aminopyroglutamamte motif induces a specific peptide conformation which favors the formation of one or two fine-membered chelating rings stabilizing a bent structure, with the coordination of 3N-type or 4N-type in the metallopeptide molecule involving metal bonding C-terminal of the modified peptide. It is worth noting that the copper (II) bonding does not undergo hydrolysis even at a very high pH range.