|BON, MARIE-CLAUDE - European Biological Control Laboratory (EBCL)|
|MANIANIA, JEAN NGUYA K - International Centre Of Insect Physiology And Ecology|
|OUNA, ELISABETH - International Centre Of Insect Physiology And Ecology|
|VAUGHAN, LARRY - Virginia Polytechnic Institution & State University|
|JEANNEAU, MELANIE - European Biological Control Laboratory (EBCL)|
|MERCADIER, GUY - European Biological Control Laboratory (EBCL)|
Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: 3/23/2009
Publication Date: 3/23/2009
Citation: Bon, M., Maniania, J., Ouna, E., Vaughan, L., Jeanneau, M., Mercadier, G. 2009. Multilocus sequence typing of Metarhizium anisopliae var acridum isolates as microbial agents for locust and grasshopper control. Genbank.
Technical Abstract: A growing interest in the biological control of locusts and grasshoppers (Acrididae) has led to the development of biopesticides based on naturally occurring pathogens which offers an environmentally safe alternative to chemical pesticides. However, the fungal strains which are being sought for biopesticide applications are the most virulent ones towards the targeted insects. A consortium project entitled “Development of Biopesticides for Grasshopper and Locust Control in Sub-Saharan Africa and funded by the USAID (Africa-Bureau–funded project Grant No AOT-G-00-97-00386-00) has concentrated on species belonging to the genus Metarhizium as the most promising candidate agents. The entomopathogenic potential of three isolates of Metarhizium anisopliae var acridum isolated in Africa is extensively evaluated at ICIPE. Therefore, typing accurately such isolates has been one of the latest issues addressed by the consortium project. We applied a strategy of multilocus sequence typing in order to identify and to delineate between the three isolates namely ICIPE 19, ICIPE 21 and ICIPE 22. The targeted sequences were from 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence (Genbank accession numbers FJ787311 to FJ787313); 28S ribosomal RNA gene, partial sequence (Genbank accession numbers FJ787314 to FJ787316); translation elongation factor 1 alpha (TEF) gene, partial sequence (Genbank accession numbers FJ787317 to FJ787319); RNA polymerase II largest subunit (RPB1) gene, partial sequence (Genbank accession numbers FJ787320 to FJ787322); RNA polymerase II second largest subunit (RPB2) gene, partial sequence (Genbank accession numbers FJ787323 to FJ787325).