Submitted to: Animal Genetics International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 6/24/2008
Publication Date: N/A
Technical Abstract: Our goal is use the Swine Protein-Annotated Oligonucleotide Microarray (www.pigoligoarray.org) to identify immune regulatory and protective pathways to uncover genetic components involved in early immune responses during porcine reproductive and respiratory syndrome virus (PRRSV) infection. Animals were divided into three groups: (1) pigs infected with a virulent PRRSV isolate MNW2B; (2) pigs vaccinated using a contemporary PRRS ATP vaccine (Ingelvac®); and (3) control pigs. Tissues [cranial lung, distal lung, tracheobronchial lymph nodes (TBLN)] were collected between days 3-6 post infection/vaccination. Total RNA was labeled using Alexa Fluor® 555 and 647 dyes (Invitrogen) and a microarray loop design applied to compare gene expression between individuals from all three groups. Validation of the pigoligoarrays was performed by analyzing transcriptional profiles for TBLN, Lung and longissimus dorsi muscle from normal or PRRSV infected pigs as confirmed using QPCR for candidate genes. Diagnostic analyses used designed array control features, negatives and perfect match/mismatch oligonucleotide sets, to assess non-specific hybridization. Final statistical analyses of the PRRSV study are underway. These studies provide important support for our PRRS Host Genetic Consortium studies of neonatal pig resistance to infection that have just begun. Overall, these studies will reveal immune pathways and candidate genes involved in PRRSV resistance.