Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: 7/2/2008
Publication Date: 7/19/2008
Citation: Davis, J., Spackman, E., Kulkarni, A., Briese, T. 2008. Identification of viruses present in tissues collected from chickens with hypoglycemia-spiking mortality syndrome (H-SMS) [abstract]. American Association of Avian Pathologists Annual Meeting, July 19-23, 2008, New Orleans, Louisiana. 2008 CDROM.
Technical Abstract: Tissues were collected, over a 10-year period, from broiler chickens diagnosed with severe H-SMS at the Georgia Poultry Lab, in Oakwood, GA. All samples were stored in tissue culture media, with antibiotics and 15% fetal bovine serum, in an ultra-cold freezer @ -80F. Specimens were homogenized, centrifuged, then filtered through 0.45micron filters, and inoculated into yolk sacs of embryonating SPF chicken eggs. Embryos which died between 72-120 hours post-inoculation were harvested and submitted for molecular testing. Results are summarized in Table 1. No specific virus could be incriminated as the sole etiologic agent of H-SMS. However, the samples which had previously been used to experimentally reproduce H-SMS contained both Chicken Rotavirus and Infectious Bronchitis Virus.