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Title: Selection of markers for mapping and cloning disease resistance in common bean

item Miklas, Phillip - Phil

Submitted to: Bean Improvement Cooperative Annual Report
Publication Type: Abstract Only
Publication Acceptance Date: 3/24/2008
Publication Date: 4/21/2008
Citation: Talukder, Z.I., Schmidt, K., Anderson, E.L., Miklas, P.N., Gonnella, T.P., Hossain, K.G. 2008. Selection of markers for mapping and cloning disease resistance in common bean. Bean Improvement Cooperative Annual Report. 51:94-95.

Interpretive Summary:

Technical Abstract: Infestation of diseases is one of the major constraints of subsistence production and economic yield of common bean. Genetic resistance is an important component of integrated strategies to combat problematic diseases in common bean and development of cultivars with improved resistance to pest and diseases is a primary goal of bean breeding programs throughout the world. Several classes of resistance genes encode proteins containing leucine-rich repeats (LRR). Amino acid sequences for a number of these resistance sequences contain strong similarity to nucleotide binding sites (NBS). Rivkin et al. (1999) developed primers from a conserved NBS and identified eight unique classes of disease-resistance related sequences in common bean. We have analyzed four consensus NBS-LRR common bean sequences and generated 37 molecular markers. Along with 34 SSRs and 21 markers developed from tentative consensus (TC) sequences of common bean, these markers were screened to select polymorphic markers among parents of seven mapping populations segregating for different disease resistance traits.Forty-nine percent (49%) of these primers were polymorphic across different parental combination and markers developed from TCs were found to be highly polymorphic (76%) followed by SSRs (65%), and NBS-LRR types (27%). The primer pairs that exhibited polymorphism among different mapping populations are presented in Table 2 with their sequences and linkage group location. The map location of NBS-LRR type markers has yet to be determined. These primers showed varying degrees of polymorphism across different mapping populations. Three primers showed polymorphism across all seven mapping populations and a maximum of 12 primers showed polymorphism in 3 mapping populations. Information generated will be useful for generating genetics maps and tagging disease resistance traits in common bean.