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Title: Optimization of strawberry volatile sampling by odor representativeness

item Plotto, Anne

Submitted to: Florida State Horticultural Society Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/5/2008
Publication Date: 4/23/2008
Citation: Jouquand, C., Plotto, A., Chandler, C. 2008. Optimization of strawberry volatile sampling by odor representativeness. Florida State Horticultural Society Meeting. Paper No. HP1.

Interpretive Summary:

Technical Abstract: The aim of this work was to choose a suitable sampling headspace technique to study 'Festival' aroma, the main strawberry cultivar grown in Florida. For that, the aromatic quality of extracts from different headspace techniques was evaluated using direct gas chromatography-olfactometry (D-GC-O), a special tool which is dedicated to assessing global odor from solvent-free extracts. A gas chromatography equipped with a sniffing port was equipped with a 15-cm deactivated silica column, thus allowing evaluation of global odor of headspace extracts without chromatographic separation. Two SPME extracts, with different types of fiber (PDMS/DVB fiber and DVD/Car/PDMS fiber), and two static headspace extracts, with different phase ratios (ratio of gas and sample phase volumes), from 'Festival' puree were evaluated using D-GC-O. A similarity test with three panelists allowed comparison of the odor of these extracts to that of 'Festival' puree. The results indicated that SPME extracts with a DVD/Car/PDMS fiber generated the most representative odor with a "green" impression and a strong "fruity" note. A fruity note was also obtained with static headspace extracts which exhibited the highest volatile concentration in headspace, i.e the lowest phase ratio. When comparing the aroma profile of each extract, the "DVD/Car/PDMS" extract showed the highest concentration of methyl butanoate and ethyl butanoate, two key compounds in strawberry aroma.