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Title: Deerberry Fruit Extracts Inhibit Activator protein-1, Nuclear Factor-kappaB and Cell Proliferation, and Induce Apoptosis by Perturbing the Mitogenic Signaling Pathway in vitro in Culture Cells

Author
item Wang, Shiow
item FENG, RENTIAN
item BOWMAN, LINDA
item LU, YONGJU
item DING, MIN
item BALLINGTON, JAMES

Submitted to: Acta Horticulture Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 5/8/2008
Publication Date: 3/1/2009
Citation: Wang, S.Y., Feng, R., Bowman, L., Lu, Y., Ding, M., Ballington, J. 2009. Deerberry Fruit Extracts Inhibit Activator protein-1, Nuclear Factor-kappaB and Cell Proliferation, and Induce Apoptosis by Perturbing the Mitogenic Signaling Pathway in vitro in Culture Cells. In: Acta Horticulture Proceedings. International Society for Horticultural Science 9th International Vaccinium Symposium, July 13-16, 2008, Corvallis, Oregon.p.149.

Interpretive Summary:

Technical Abstract: Fruit of deerberry [Vaccinium stamineum L.] were evaluated for their antioxidant capacity, antioxidant enzyme activity and anti-cancer properties in JB6 P+ mouse epidermal cells and human leukemia (HL-60) cells. Deerberries contain potent free radical scavenging activities and also had high activities of antioxidant enzymes. Pretreatment of JB6 P+ mouse epidermal cells with deerberry fruit extracts produced an inhibition on the activation of activator protein-1 (AP-1) and nuclear factor-kappaB (NF-'B) induced by either 12-O-tetradecanoylphorbol-13-acetate (TPA) or ultraviolet-B (UVB). Deerberry fruit extracts also blocked TPA- or UVB-induced phosphorylation of ERKs and MEK ½, two upstream regulators of AP-1 and inhibited proliferation of human leukemia HL-60 cancer cells and induced apoptosis of HL-60 cells. These results suggest that the inhibition of TPA- or UVB-induced AP-1 and NF-'B activity, inhibition of HL-60 cells proliferation and induction of apoptosis in human leukemia HL-60 cancer cells by deerberry fruit extracts may be mediated through the ERKs and MEK ½ signal pathway.