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Title: Deerberry Fruit Extracts Inhibit Activator protein-1, Nuclear Factor-kappaB and Cell Proliferation, and Induce Apoptosis by Perturbing the Mitogenic Signaling Pathway in vitro in Culture Cells

Author
item Wang, Shiow
item FENG, RENTIAN - NIOSH, CDC, MORG, WVA
item BOWMAN, LINDA - NIOSH, CDC, MORG, WVA
item LU, YONGJU - NIOSH, CDC, MORG, WVA
item DING, MIN - NIOSH, CDC, MORG, WVA
item BALLINGTON, JAMES - NCSU, RALEIGH, NC

Submitted to: Acta Horticulture Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 5/8/2008
Publication Date: 3/1/2009
Citation: Wang, S.Y., Feng, R., Bowman, L., Lu, Y., Ding, M., Ballington, J. 2009. Deerberry Fruit Extracts Inhibit Activator protein-1, Nuclear Factor-kappaB and Cell Proliferation, and Induce Apoptosis by Perturbing the Mitogenic Signaling Pathway in vitro in Culture Cells. In: Acta Horticulture Proceedings. International Society for Horticultural Science 9th International Vaccinium Symposium, July 13-16, 2008, Corvallis, Oregon.p.149.

Interpretive Summary:

Technical Abstract: Fruit of deerberry [Vaccinium stamineum L.] were evaluated for their antioxidant capacity, antioxidant enzyme activity and anti-cancer properties in JB6 P+ mouse epidermal cells and human leukemia (HL-60) cells. Deerberries contain potent free radical scavenging activities and also had high activities of antioxidant enzymes. Pretreatment of JB6 P+ mouse epidermal cells with deerberry fruit extracts produced an inhibition on the activation of activator protein-1 (AP-1) and nuclear factor-kappaB (NF-'B) induced by either 12-O-tetradecanoylphorbol-13-acetate (TPA) or ultraviolet-B (UVB). Deerberry fruit extracts also blocked TPA- or UVB-induced phosphorylation of ERKs and MEK ½, two upstream regulators of AP-1 and inhibited proliferation of human leukemia HL-60 cancer cells and induced apoptosis of HL-60 cells. These results suggest that the inhibition of TPA- or UVB-induced AP-1 and NF-'B activity, inhibition of HL-60 cells proliferation and induction of apoptosis in human leukemia HL-60 cancer cells by deerberry fruit extracts may be mediated through the ERKs and MEK ½ signal pathway.