Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 11/1/2006
Publication Date: 1/1/2007
Citation: Reddy, L.J., Ling, P., Wang, M.N., Chen, X., Garland Campbell, K.A. 2007. Isolation of full-length candidate CBF genes from hexaploid wheat (Triticum aestivum L.). Plant and Animal Genome XV, Jan 13-17, 2007, San Diego, CA. pg 116.
Technical Abstract: Cold temperature is a serious abiotic stress to cereals in areas with severe winters. CBF (C-repeat binding factor) is a transcription factor that regulates Cold regulated (COR) genes in Arabidopsis and it is an important component of the CBF cold acclimation pathway. The CBF gene family is hypothesized to play an important role in cold tolerance in cereals. In this study, ortholog sequences of TmCBF (Triticum monococcum) and HvCBF (Hordeum vulgare ) genes and one EST sequence from bread wheat (Triticum aestivum L) were used to generate screening primers to isolate full length candidate CBF genes from hexaploid wheat. Hexaploid wheat BAC library constructed in our unit for cloning disease resistance gene was used in this study. The six dimensional BAC library pools were screened with the ortholog markers to isolate CBF gene candidate clones. Three primer pairs were able to efficiently identify BAC clones containing CBF candidate alleles. Selected candidate BAC clones were confirmed and sequenced for full length CBF gene structure analysis. Cloning of CBF ortholog loci from hexaploid wheat will reveal gene structure and sequence information that may be related to cold tolerance in wheat which can be utilized for direct cold tolerance marker development and genetic manipulation for crop improvements.