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Title: Genetic variations of 14-3-3E1 isoform in rainbow trout (Oncorhynchus mykiss)

item Rexroad, Caird

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/12/2008
Publication Date: 1/12/2008
Citation: Wanna, W., Rexroad III, C.E., Jianbo, Y. 2008. Genetic variations of 14-3-3E1 isoform in rainbow trout (Oncorhynchus mykiss). Plant and Animal Genome Conference.

Interpretive Summary:

Technical Abstract: The highly conserved family of 14-3-3 proteins functions in the regulation of a wide variety of cellular processes. The presence of 14-3-3 isoforms and the diversity of cellular processes regulated by 14-3-3 isoforms suggest functional specificity of the isoforms. Several studies have observed differences in affinity and functionality of 14-3-3 isoforms. However, the structural variation by which isoform specificity is accomplished remains unclear. In rainbow trout, ten 14-3-3 genes which are likely to be duplicates of five ancestral genes have been reported. In this study, we examined the genetic variations of the 14-3-3E1 gene in rainbow trout. The open reading frame (ORF) of the 14-3-3E1 gene was amplified from a pool of cDNAs derived from 10 different rainbow trout tissues and cloned into pCRII vector. A total of 14 random clones were sequenced. DNA sequence analysis showed substantial sequence variations in the ORF of the 14-3-3E1 gene. A number of missense mutations were identified that result in several single amino acid substitutions. Four of the clones showed a deletion of 3 nucleotides (CAG), resulting in the deletion of Alanine at position 239. Interesting, two sequences showed an insertion of 45 nucleotides that results in the introduction of a premature stop codon. This insertion mutation was confirmed by PCR detection of a larger fragment. The presence of numerous mutations in the coding region of the 14-3-3E1 gene is an important indication of the diverse functions of this protein.