Submitted to: Physiological and Molecular Plant Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/11/2008
Publication Date: 6/25/2008
Citation: Larson, R.L., Wintermantel, W.M., Hill, A.L., Fortis, L.L., Nunez, A. 2008. Proteome changes in sugar beet in response to Beet necrotic yellow vein virus. Physiological and Molecular Plant Pathology. doi:10.1016/j.pmpp.2008.04.003
Interpretive Summary: Rhizomania, caused by Beet necrotic yellow vein virus (BNYVV), is a problem for sugar beet producers worldwide. Major sugar losses are noted with this disease, since the main sugar storage site, the taproot, is constricted by overgrowth of the lateral roots. This change in root morphology, symptomatic of the disease, is commonly called “hairy root”. There are only a few characterized sources of natural resistance in sugar beet effective against this pathogen and in recent years, some resistance sources have become ineffective against certain isolates of the virus. By examining protein changes in sugar beet roots susceptible or resistant to BNYVV following infection we are gaining a better understanding of the mechanisms behind disease development and resistance. This knowledge allows for development of more effective disease management strategies and creation of new methods for more rapid and accurate selection of new sources of resistance in sugarbeet.
Technical Abstract: Beet necrotic yellow vein virus (BNYVV) is a devastating sugarbeet pathogen. Resistance is limited and resistance-breaking isolates are becoming problematic. Effective disease control strategies will come from a better understanding of disease and resistance. BNYVV-induced differential sugarbeet protein expression was evaluated with multidimensional liquid chromatography. Of more than 1,000 protein peaks detected in root extracts, 7.4 and 11% were affected by BNYVV in the resistant and susceptible genotype, respectively. Using tandem MALDI-TOF-MS, 65 proteins were identified; differential expression of some was verified using RT-PCR. Proteomic data suggest involvement of systemic resistance components in Rz1-mediated resistance and phytohormones in symptom development.