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United States Department of Agriculture

Agricultural Research Service

Title: A phenylalanine in DGAT is a key determinant of oil content and composition in maize)

Author
item Zheng, Peizhong
item Allen, William
item Roesler, Keith
item Williams, Mark
item Zhang, Shirong
item Li, Jiming
item Glassman, Kimberly
item Ranch, Jerry
item Nubel, Douglas
item Solawetz, William
item Bhattramakki, Dinakar
item Llaca, Victor
item Deschamps, Stephane
item Zhong, Gan-yuan
item Tarczynski, Mitchell
item Shen, Bo

Submitted to: Nature Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/4/2007
Publication Date: 2/17/2008
Citation: Zheng, P., Allen, W., Roesler, K., Williams, M., Zhang, S., Li, J., Glassman, K., Ranch, J., Nubel, D., Solawetz, W., Bhattramakki, D., Llaca, V., Deschamps, S., Zhong, G., Tarczynski, M., Shen, B. 2008. A phenylalanine in DGAT is a key determinant of oil content and composition in maize. Nature Genetics. 40:367-372.

Interpretive Summary: Plant oil is an important renewable energy source for human consumption and industrial use, especially for biodiesel production. Maize kernel oil content is controlled by multiple quantitative loci (QTLs). Here we report the fine-mapping and positional cloning of an acyl-CoA: diacylglycerol acyltransferase (DAGT1-2) gene responsible for a major oil/oleic QTL located on maize chromosome six. In addition, we demonstrate that a phenylalanine (F469) in DAGT1-2 plays a crucial role in controlling oil and oleic contents.

Technical Abstract: Plant oil is an important renewable energy source for human consumption and industrial use, especially for biodiesel production. Maize kernel oil content is controlled by multiple quantitative loci (QTLs). The molecular basis of oil QTL, however, remains unknown. Here we report that the qHO6 gene, a major QTL affecting kernel oil and oleic content, encodes an acyl-CoA: diacylglycerol acyltransferase (DAGT1-2) and demonstrate that a phenylalanine (F469) in expression of high oil DAGT1-2 increased kernel oil content by as much as 41%.

Last Modified: 05/26/2017
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