Submitted to: Bulletin of Insectology
Publication Type: Abstract Only
Publication Acceptance Date: 8/15/2007
Publication Date: 12/1/2007
Citation: Wei, W., Lee, I., Davis, R.E., Suo, X., Zhao, Y. 2007. Virtual RFLP analysis of 16S rDNA sequences identifies new subgroups in the clover proliferation phytoplasma group. Bulletin of Insectology. 60:349:350.
Technical Abstract: Phytoplasmas are insect-transmitted, phloem-inhabiting, cell wall-less bacteria that cause numerous diseases in several hundred plant species. In adaptation to diverse plant hosts and insect vectors, phytoplasmas have evolved to give rise to widely divergent lineages. As phytoplasmas are unculturable, molecular analyses of conserved gene sequences have become a rational means for phytoplasma differentiation and classification. Extending our recent effort in exploitation of computer-simulated 16S rDNA RFLP analysis and virtual gel plotting for rapid classification of phytoplasmas, we have developed an algorithm for automated RFLP pattern comparison and similarity coefficient calculation. Such streamlined virtual RFLP pattern analysis of 16S rDNA sequences has led to identification of new pattern types and potential new subgroups in the clover proliferation phytoplasma group.