Submitted to: World Veterinary Poultry Association
Publication Type: Abstract Only
Publication Acceptance Date: 6/14/2007
Publication Date: 9/10/2007
Citation: Fadly, A.M., Mays, J.K., Silva, R.F., Lee, L.F. 2007. Characterization of reticuloendotheliosis virus isolates obtained from chickens, turkeys and prairie chickens in the United States [abstract]. 2007 Beijing 15th Congress World Veterinary Poultry Association Abstract Book. p. 208.
Technical Abstract: Twelve reticuloendotheliosis virus (REV) isolates obtained from chickens, turkeys and prairie chickens in the United States were characterized using ploymerase chain reaction (PCR) and indirect immunofluoresence (IFA) assays. This study included five REV isolates from Prairie chickens in Texas, two REV isolates from broiler breeders in Alabama, three REV isolates from chickens in Maine and two REV isolates from turkeys in California. All isolates were propagated in chicken-embryo fibroblasts (CEFs) obtained from a specific-pathogen-free (SPF) breeder flock. Proviral DNA obtained from CEFs inoculated with each of REV isolates was analyzed by PCR using primer sets that amplify the 291 base pairs product of REV long terminal repeat (LTR). To determine subtype of the REV isolate, inoculated CEFs grown on microtiter plates were tested by an IFA using REV monoclonal antibodies, 11D182, 11C237 and 11B118. Results indicated that all isolates were successfully propagated in SPF CEFs used in this study, suggesting no differences in the ability of these isolates to grow in CEFs. PCR analysis of all twelve isolates resulted in the amplification of the 291 base pairs REV LTR; none of the isolates exhibited a different pattern or shift from the expected PCR product of REV LTR. The subtype of these REV isolates originally obtained from four different States in the USA, and whether they are related to each other or to the prototype strain of REV subtypes, A, B, or C will be discussed.