|Meinersmann, Richard - Rick|
Submitted to: Campylobacter Helicobacter and Related Organisms International Workshop
Publication Type: Abstract Only
Publication Acceptance Date: 6/7/2007
Publication Date: 9/1/2007
Citation: Meinersmann, R.J., Ladely, S.R. 2007. Inter- and intra-genomic heterogeneity of the intervening sequence in the 23S ribosomal RNA gene of Campylobacter jejuni and Campylobacter coli. Campylobacter Helicobacter and Related Organisms International Workshop. 54(Supp1):64.
Technical Abstract: An intervening sequence (IVS) can be present or absent in the 23S rRNA of Campylobacter jejuni and C. coli. Previously (CHRO 2005) we presented data indicating heterogeneity in the presence of the 23S rRNA IVS within the operons of some isolates. Nested PCR reactions with operon-specific primers followed by primers for the IVS confirmed that the strains of interest contained either one or two operons carrying the IVS and the remaining operon(s) did not. Sequence analysis of the IVS and flanking regions of the 23S rRNA genes did not discriminate C. jejuni and C. coli as distinct populations. These results may indicate horizontal transfer of 23S rRNA genes or portions of the gene between C. jejuni and C. coli. Also, data showing sequence polymorphisms between the three 23S rRNA loci outside of the IVS region suggest that the isolates with intragenomic heterogeneity appear to be members of clones that have an ancient defect in gene conversion mechanisms needed for concerted evolution of the ribosomal operons.