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ARS Home » Northeast Area » Geneva, New York » Plant Genetic Resources Unit (PGRU) » Research » Publications at this Location » Publication #209923

Title: Computational Reclassification of Resistance Gene Analogs in Rosaceae Based on the NBS-LRR Domain

item Baldo, Angela

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 12/1/2006
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Plant R genes are known to confer resistance to a variety of pathogens in a gene-for-gene mode. One hundred and ninety-five Rosaceae putative resistance gene analog (RGA) sequences containing conserved NBS-LRR domains were downloaded from the public domain. An additional seventy-six RGAs were amplified and sequenced from red raspberry Rubus idaeus cv “Latham” using degenerate primers. Sequences were aligned using Hidden Markov Models and clustered with Neighbor-Joining. There are eleven TIR and ten non-TIR sequence clusters containing two or more genera with a bootstrap greater than 50%. This classification will be used to help identify novel RGAs found in the USDA-ARS apple and cherry germplasm collections in Geneva, NY.