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ARS Home » Northeast Area » Geneva, New York » Plant Genetic Resources Unit (PGRU) » Research » Publications at this Location » Publication #209834

Title: SNP Validation and Genetic Diversity in Cultivated Tomatoes and Grapes

item Baldo, Angela
item Lamboy, Warren
item Simon, Charles
item Labate, Joanne
item Srmack, Susan

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 12/1/2006
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Cultivated grapes and tomatoes have very different reproductive systems. While grapes are often outcrossed and grafted, tomatoes are generally selfed and propogated by seed. Large-scale public EST datasets were used in both crops to predict SNPs and PCR primers flanking these SNPs. Genomic DNA was amplified and the gene fragments were resequenced. These results were analyzed in each crop for the distribution of genetic diversity among cultivars and within genes. Frequency of intron discovery, SNP validation rates, and proportions of rare alleles reflect the different propogation histories of these crops.