Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2007
Publication Date: N/A
Technical Abstract: Biotrophic pathogens rely upon components of host machinery for their survival and propagation. Natural or induced mutation of host machinery components has repeatedly been harnessed to obtain applicable and durable resistance, as exemplified by recessive eIF4E and mlo resistances. In this project, random EMS mutagenesis was applied to Vitis vinifera ‘Chardonnay,’ and a population of 100,000 M2 seeds was generated. In screening the first 5,000 seedlings, individuals with resistance to powdery mildew or downy mildew were identified, but no individuals have been identified with resistance to both diseases. Field resistance to downy mildew was tested in a separate population of 955 seedlings, and four individuals were identified as resistant. The M2 Chardonnay population has value for assaying other resistances or phenotypes. In a complimentary approach, TILLING (Targeting Induced Local Lesions IN Genomes) is being used to identify mutations in candidate genes for functional analysis. Progress with TILLING in the highly heterozygous grapevine genome will be discussed and resistance results described.