|Miklas, Phillip - Phil|
Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: 11/1/2006
Publication Date: 1/12/2007
Citation: Anderson, E.L., Miklas, P.N., Dudwadkar, G.P., Gonnella, T.P., Hossain, K.G. 2007. Disease Resistance Gene homologs in Common Bean. Plant and Animal Genome. San Diego, Jan. 10-15, 2007.
Technical Abstract: The ever increasing numbers of publicly available genomic database resources of important plant and animal species have opened up the scope of utilizing genomic information of one species into other species. The common bean consisting of dry and snap beans, is one of the most important species of leguminous crops but there are many diseases that significantly reduce the persistence and productivity of this species in the US and worldwide. Due to the lack of adequate genomic resources, bean breeders and growers across the world have been suffering from a shortage of functional markers for tagging and monitoring disease resistance traits in this species. Searing the genomic database resources of legumes, we have identified over 100 disease resistance gene sequences. PCR primers from these sequences have been used to amplify DNA from different breeding lines of common bean segregating for disease resistance traits. In our preliminary analysis with 36 primers, about 50% of the primers amplified common bean genome, of which 66% produced single product with minimum PCR optimization, and 5% polymorphic in different parental combinations. Amplification of the primers will be further evaluated using higher resolution gel electrophoresis (6% non-denaturing gel electrophoresis) and single PCR products will be sequenced to identify resistance gene homologs in common bean and molecular markers like SSR, SNP, etc. This project will help in establishing a strong foundation in research in common bean genomics and generating data for molecular markers for disease resistance in common bean.