Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: 10/15/2006
Publication Date: 1/2/2007
Citation: Ling, P., Wang, M.N., Chen, X. 2007. Isolation of genes underlining important functions of wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici). Plant and Animal Genome XV, Jan 13-17, 2007, San Diego, CA, pg 117.
Technical Abstract: Puccinia striiformis f. sp. tritici causes stripe rust, one of the most destructive diseases of wheat worldwide. To determine sequences and genomic architectures of important genes for the fungal pathogen, we have isolated genomic clones containing previously identified full-length cDNA sequences encoding genes for the fungal pathogenicity and development. The full-length cDNA sequences representing genes identified from the NCBI fungal database included elongation factor, conidiation protein, transaldolase, serine/threonine kinase receptor, beta tubulin, differentiation-related protein, chitinase-like protein, and iron-sulfur cluster isul-like protein. Gene-specific markers that were developed for 12 selected full-length cDNA clones were used to screen a BAC library of the pathogen using a six-dimensional pool approach. A total of 349 positive BAC clones were identified. These BAC clones, will be fingerprinted to determine the physical relationship of these genes. Information of the sequences and structures of the selected genomic clones should provide a better understanding of the host-pathogen interactions and molecular markers for studying the pathogen population structures.