Submitted to: Society of Industrial Microbiology Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/2/2007
Publication Date: 5/2/2007
Citation: Liu, S., Skinner-Nemec, K.A., Leathers, T.D. 2007. A novel Lactobacillus buchneri strain converts a concentrated mixture of C5 and C6 sugars into ethanol and other products [abstract]. Society of Industrial Microbiology Annual Meeting. p. 158.
Technical Abstract: Lactobacillus buchneri strain NRRL B-30929 was isolated from a fuel ethanol production facility. This heterofermentative, facultative anaerobe can utilize xylose as a sole carbon source and tolerates up to 12% ethanol. Carbohydrate utilization (API, Biomerieux) and Phenotype Microarrays™ (PM, Biolog) analyses indicated that the strain is able to metabolize a broad spectrum of carbon sources, including various monosaccharides (C5 & C6), disaccharides, and oligosaccharides. More substrates were used under anaerobic than aerobic conditions. In pH-controlled bioreactors, NRRL B-30929 fermented glucose alone (10%, pH 4.0) to lactate (240 mM), ethanol (232 mM), and acetate (57 mM). This strain also utilized high concentrations of xylose and glucose simultaneously (12% each, pH 6.0) to produce lactate, acetate, and ethanol. Another interesting product recovered in the fermentation broth is a 3.5 kDa peptide which was identified as buchnericin. The simultaneous production of lactate, ethanol, and buchnericin from lignocellulosic biomass has potential application in bio-based refinery platforms.