|KIM, BEOM SOO|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/8/2006
Publication Date: 12/8/2006
Citation: Kim, B., Song, B., Hou, C.T. 2006. Production of lipase and oxygenated fatty acids from vegetable oils [abstract]. International Symposium on Biocatalysis and Bioenergy. J-4. p. 66.
Technical Abstract: More than 18 billion pounds of soybean oil is produced annually in the United States with a yearly carryover of more than 300 million pounds. How to utilize this surplus oil effectively becomes a large economic issue in the U.S. agricultural community. Soybean oil is a relatively cheap raw material and an attractive candidate for bioindustries. The content of unsaturated fatty acids such as oleic and linoleic acids are 22% and 55% for soybean oil and 26% and 60% for corn oil, respectively. Lipase produced by Candida cylindracea has been one of the most widely used enzymes in research due to its high activity in hydrolytic reactions as well as synthetic chemistry. The keto and hydroxy group gives a fatty acid special properties, such as higher viscosity and reactivity compared with other fatty acids. Keto- and hydroxy- fatty acids are useful industrial chemicals used in plasticizers, surfactants, lubricants and detergent formulations because of their special chemical attributes. In this study, oleic acid was converted to produce two value-added products, extracellular lipase and 10-ketostearic acid (10-KSA), using C. cylindracea and Flavobacterium sp. strain DS5, respectively. The effects of environmental factors in flask cultures and improvement in product concentration by high cell density fed-batch cultures will be reported.