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Title: Development of a real-time RT-PCR assay for turkey cytokines

item Day, James
item Spackman, Erica

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: 5/2/2007
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Recent pathogenesis studies with turkey-origin reoviruses (TRVs) in specific pathogen free (SPF) poults have revealed evidence of immuosuppression associated with TRV infection. The ability to quantitatively measure the abundance and/or relative amounts of cytokine mRNA in poult immune system tissue (i.e., bursa, spleen) would greatly enhance our ability to understand the immunosuppression caused by the TRVs and other disease agents. To this end, a real-time RT-PCR assay was developed for the turkey cytokines IL-1beta, IL-2, IL-18, and INF-gamma. This assay will prove useful in understanding the role cytokines play in the turkey immune response to viral pathogens.