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Title: Sequencing and mapping of bovine toll-like receptor-4 (TLR-4) signaling molecules

item Connor, Erin
item Bannerman, Douglas

Submitted to: Conference Research Workers Disease Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 10/30/2006
Publication Date: 12/3/2006
Citation: Cates, E.A., Connor, E.E., Williams, J.L., Bannerman, D.D. 2006. Sequencing and mapping of bovine toll-like receptor-4 (TLR-4) signaling molecules. Proceedings of the 87th Annual Meeting of the Conference of Research Workers in Animal Diseases. p. 116, Dec 3-5, 2006, Chicago, IL.

Interpretive Summary:

Technical Abstract: Toll-like receptor (TLR)-4 is a transmembrane receptor for lipopolysaccharide, a highly pro-inflammatory component of the outer membrane of Gram-negative bacteria. To date, molecules of the TLR-4 signaling pathway have not been well characterized in cattle. The goal of this study was to clone and sequence the full-length coding regions of bovine genes involved in TLR-4 signaling including CASP8, IRAK1, LY96 (MD-2), TICAM2, TIRAP, TOLLIP, and TRAF6 and to position these genes, as well as MyD88 and TICAM1, on the bovine genome using radiation hybrid mapping. Results of this work indicate differences with a previously published bovine sequence for LY96 and a predicted sequence in the GenBank database for TIRAP based on the most recent assembly of the bovine genome. In addition, discrepancies between actual and predicted chromosomal map positions based on the Btau_2.0 genome assembly release were identified, although map positions were consistent with predicted locations based on the current bovine-human comparative map. Alignment of the bovine amino acid sequences with human and murine sequences showed a broad range in conservation, from 52 to 93%. Overall, this work should assist in the assembly and annotation of the bovine genome sequence, the identification of variations in genes critically involved in host innate immunity, and facilitate the study of TLR-4 signaling pathways in cattle.