Submitted to: Conference Research Workers Disease Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 10/9/2006
Publication Date: 12/3/2006
Citation: Tabatabai, L.B., Zimmerli, M.K., Zehr, E.S., Briggs, R.E., Tatum, F.M. 2006. The CHAPS-soluble outer membrane proteome of Pasteurella multocida [abstract]. Conference Research Workers Disease Meeting. Paper No. 15.
Technical Abstract: Fowl cholera continues to be of major concern to the poultry industry, especially for turkey growers. This disease costs the turkey industry millions of dollars annually. Fowl cholera is caused primarily by three serotypes of Pasteurella multocida serotypes A:1, A:3, and A:4. a live attenuated vaccine strain, strain M-9 (serotype 3,4) has been implicated in outbreaks associated with vaccination. In 2001, Rimler identified an outer membrane-associated protein with a molecular mass of 39,000 that was believed to be the cross protective factor and was later characterized by Tabatabai and Zehr (2004), using 1D-gels using peptide mass finger printing and C-18 HPLC purification of two tryptic peptides. We used similar methods to identify the remainder of the 11 bands in the 1-D gel of the CHAPS-extracted insoluble residue that was solubilized in sodium dodecyl sulfate before electrophoresis. Only four additional proteins could be identified, for a total of 9 out of 11 proteins present in the membrane extract. The bands included a Putative Virulence Factor (Accession no. 15603684), LipA (Accession no. 15602638), HemY (Accession no. 15603680), and Unknown lipoprotein (Accession No. 15602915). Both the Putative Virulence Factor and the HemY protein will be further explored as virulence factors for P. multocida.