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Title: Isolation and characterizaton of dinucleotide microsatellites in greater amberjack, Seriola dumerili

item Rexroad, Caird

Submitted to: Conservation Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/30/2007
Publication Date: 9/14/2007
Citation: Renshaw, M.A., Patton, J., Rexroad III, C.E., Gold, J. 2007. Pcr primers for dinucleotide microsatellites in greater amberjack, seriola dumerili. Conservation Genetics 2007, 8(4):1009-1011.

Interpretive Summary: Greater amberjack, Seriola dumerili, is an economically important, reef-associated carangid fish with a circumglobal distribution in sub-tropical and temperate waters. In U.S. waters, increased commercial and recreational fishing pressure on this species has led to establishment of fishery limitations along the Atlantic coast and an assessment of the species as ‘overfished’ in the northern Gulf of Mexico. Additionally, there is worldwide interest in commercial aquaculture of greater amberjack due to its relatively rapid growth rate and excellent market potential. We report development of 13 genetic markers for (i) providing population-genetic data to assist with management of ‘wild’ stocks, and (ii) applications in aquaculture investigations, including assessment of genetic variability and inbreeding, parentage assignment, and quantitative trait loci (QTL) identification.

Technical Abstract: Thirteen nuclear-encoded dinucleotide microsatellites were characterized from a genomic DNA library of greater amberjack, Seriola dumerili. The microsatellites include 12 perfect-repeat motifs and one imperfect-repeat motif. The number of alleles at the 13 microsatellites among a sample of 29 fish ranged from three to 25; gene diversity (expected heterozygosity) ranged from 0.296 to 0.948, while observed heterozygosity ranged from 0.276 to 0.897. Following Bonferroni correction, genotypes at all thirteen microsatellites fit expectations of Hardy-Weinberg equilibrium. One pairwise comparison of microsatellites deviated significantly from expectations of genotypic equilibrium, suggesting that these two microsatellites may be linked. Greater amberjack support commercial and recreational fisheries along both the Atlantic and Gulf coasts of the U.S. and represent a species with potential for worldwide aquaculture. The microsatellites developed will be useful for population genetic studies of ‘wild’ populations and breeding studies of domesticated populations.