Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 9/1/2005
Publication Date: 1/1/2006
Citation: Yan, G.P., Chen, X. 2006. Molecular mapping of a gene for resistance to stripe rust in bba 2890 barley. Plant and Animal Genome Abstracts. Page 186. Jan. 14-18, 2006, San Diego, CA.
Technical Abstract: Stripe rust, caused by Puccinia striiformis f. sp. hordei (PSH), is an important disease on barley. Growing resistant cultivars is the best approach for controlling the disease. Barley genotype BBA 2890 has all-stage (also known as seedling) resistance against all PSH races identified thus far in the U. S. The resistance in BBA 2890 was controlled by a single recessive gene, designated as rps1.a. To develop a molecular map for the gene, a cross was made between BBA 2890 and susceptible Steptoe, and a population of 200 F8 recombinant inbred lines (RILs) were developed through single-seed-descent. Seedlings of the parents and all F8 RILs were tested for resistance to races PSH-14, PSH-48, and PSH-54 under controlled greenhouse conditions. Genomic DNA was extracted from the parents and 150 F8 RILs. The resistance gene analog polymorphism (RGAP) technique was used to identify molecular markers for the rps1.a gene. Out of 267 primer pairs screened, 52 produced polymorphic bands in the two parents and also in the bulk segregant analysis. Six primer pairs generating repeatable polymorphic bands were selected for linkage analysis with the 150 F8 RILs. A genetic linkage group was constructed for the resistance gene with seven RGAP markers produced by the six primer pairs. The closest marker for the resistant allele was 6.4 cM away from the locus. The closest marker for the susceptible allele was 12.4 cM away from the locus. Chromosomal-specific simple sequence repeat (SSR) markers are being used to map the gene on a barley chromosome.