Submitted to: Eastern Analytical Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 7/24/2006
Publication Date: 10/12/2006
Citation: Kays, S.E., Kim, Y., Singh, M. 2006. Nir spectroscopy of microwaveable meals [abstract]. 708. Eastern Analytical Symposium.
Technical Abstract: The potential of near infrared (NIR) spectroscopy for the prediction of total dietary fiber (TDF) in mixed meals was investigated. Meals were prepared for spectral analysis by homogenization only (HO), homogenization and drying (HD), and homogenization, drying and de-fatting (HDF). The NIR spectra (400-2498 nm) were obtained with a dispersive NIR spectrometer. TDF was determined in HDF samples by an enzymatic-gravimetric assay (AOAC 991.43) and values calculated for HD and HO samples. Using multivariate analysis software and optimum processing, PLS models (n=114) were developed to relate NIR spectra to the corresponding TDF values. The HO, HD, and HDF models predicted TDF in independent validation samples (n=37) with SEP of 0.93 (range 0.7-8.4) %, 1.90 (range 2.2-18.9) %, and 1.45 (range 2.8-23.3) % and r2 of 0.89, 0.92 and 0.97, respectively. Compared with traditional analysis of TDF in mixed meals, which takes 4 days, NIR spectroscopy provides a faster method for screening samples for TDF. The accuracy of prediction was greatest for the HDF model followed by the HD model.