Submitted to: Animal Genetics
Publication Type: Peer reviewed journal
Publication Acceptance Date: 9/2/2006
Publication Date: 10/2/2006
Citation: Shini, J.H., Kim, H., Lim, D., Jeon, M., Hani, B.K., Park, T.S., Kim, J.K., Lillehoj, H.S., Cho, B.W., Han, J.Y. 2006. Analysis of chicken embryonic gonad ests. Animal Genetics 37:85-86. Interpretive Summary: In this paper, ARS scientists collaborated with scientists at Seoul National University in South Korea to develop a geneomic resource from chicken embryonic gonads. Total 11,842 cDNA clones from the embryonic gonad cDNA library were sequenced and their gene sequences submitted to NCBI dbEST public database. This genetic resource will provide important sequence information and genes for scientists who study embryo gonad development in poultry.
Technical Abstract: We have sequenced 11,842 cDNA clones from the embryonic gonad cDNA library to generate 10,294 sequences. The EST data described in this paper have been submitted to the NCBI dbEST under accession numbers CV852525 CV862818. The unique sequences of the EST data resulted in a total of 4,384 sequences with 934 contigs and 3,450 singlets. The average number of ESTs per contig was about 4.3. The number of putative novel transcripts obtained by comparison with GgGI (Release 10.0) totaled 1,215 sequences with 131 contigs and 1,084 singlets. Among the putative novel transcripts, about 10% of sequences were blast best hits in chicken. Functional prediction and gene ontology distribution on the basis of sequence similarity searches against the nonredundant protein database of the NCBI. Interestingly, Pearson=s chi square test of independence of 2nd level GO terms between gonad and novel transcripts indicates varieties of extracellular proteins may be involved in the gene expression of early embryonic gonads. In addition, transporter activity, obsolete molecular functions, and behavior terms were higher in the novel transcripts than in the gonad set.