Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/23/2006
Publication Date: 5/23/2006
Citation: Frye, J.G., Cray, P.J., Jackson, C.R., Englen, M.D. 2006. Detection of exogenous antimicrobial resistance genes by dna microarray [abstract]. Symposium on DNA-based Serotyping and Subtyping of Enteric Bacteria. March 23, 2006. Atlanta, Georgia.
Technical Abstract: One factor leading to the spread of antimicrobial resistance in bacteria is the horizontal transfer of exogenous genes encoding resistance. To study this spread, it is necessary to identify the genes responsible for resistance. Currently, each gene must be screened individually in order to identify the gene(s) responsible for the observed resistance(s) expressed by a bacterium. The inability to rapidly identify these genes limits research progress. We have designed, constructed, and validated a DNA microarray to simultaneous test for all resistance genes found in the National Center for Biotechnology Information database. The microarray was also shown to successfully detect resistance genes in a variety of diverse bacteria, including Salmonella, E. coli, Camplyobacter and Enterococcus, and yielded results virtually identical to the current standard techniques of PCR and Southern blotting. This technique can be used to monitor the spread of antimicrobial resistance and allow the development of strategies to prevent it.