Submitted to: International Conference of Polyphenols
Publication Type: Proceedings
Publication Acceptance Date: 5/18/2006
Publication Date: 8/22/2006
Citation: Luthria, D.L., Mukhopadhyay, S., Krizek, D.T. Content of total phenolics and phenolic acids in tomato fruits as influenced by cultivar and ambient solar UV radiation. International Conference of Polyphenols. XXIII International Conference of Polyphenols, Aug. 22 -25, 2006, Winnipeg, Manitoba.
Technical Abstract: Two cultivars of tomato samples, Oregon spring (OS) and Red sun (RS) were grown in high tunnel which were covered with two contrasting covering materials of similar thickness (0.152mm) and durability (4-year polyethylene). One material (Dura-film Super 4) blocked UV (ultraviolet) radiation (< 380 nm) and other material (Tyco Tufflite IV) allowed UV radiation. Four sets of tomato samples were collected from each cultivar grown under two different conditions, one in presence of UV light (UV+) and other that partially blocked ultraviolet light (UV-). All sixteen samples were assayed for total phenolic (TP) content by a colorimetric Folin-Ciocalteu assay (FC) and for phenolic acid content by a high performance liquid chromatography-diode array detection (HPLC-DAD) procedure. Three phenolic acids that were extracted in the base hydrolyzed fraction of tomato samples were identified as caffeic acid, p-coumaric acid and ferulic acid. Caffeic acid was the most predominant phenolic acid in both cultivars (OS and RS) grown in two different environments. About 10-20% higher amounts of phenolic acids were extracted from both cultivars (OS and RS) grown in presence of UV light.