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Title: CONSTRUCTION OF AN ARABICA COFFEE BAC LIBRARY FOR MOLECULAR DISSECTION OF AN ALLOTETRAPLOID GENOME

Author
item JONES, M - HARC
item BYERS, A - HARC
item SKELTON, R - HARC
item YU, Q - HARC
item NAGAI, C - HARC
item Moore, Paul
item MING, R - UNIV ILLINOIS

Submitted to: International Conference on Coffee Science (ASIC)
Publication Type: Abstract Only
Publication Acceptance Date: 3/30/2006
Publication Date: 9/11/2006
Citation: Jones, M.R., Byers, A., Skelton, R.L., Yu, Q., Nagai, C., Moore, P.H., Ming, R. 2006. Construction of an arabica coffee bac library for molecular dissection of an allotetraploid genome. 21st International Conference on Coffee Science (ASIC). B211 Pg. 49.

Interpretive Summary:

Technical Abstract: Arabica coffee (C. Arabica) is commercially the most important species of the genus Coffea. Arabica accounts for 70% of the world’s coffee production. We constructed a bacterial artificial chromosome (BAC) library using genomic DNA from the small bean, high-cupping quality, Arabica variety Mokka Hybrid that was crossed with the large bean, low-cupping quality, Arabica variety Catimor to generate a segregating population for linkage and quantitative trait loci (QTL) mapping. The genomic DNA was partially digested with the restriction enzyme Hind III and ligated into the improved BAC vector pIndigo. The resultant BAC library consists of 52,416 clones with an average insert size of 94 kb. It was estimated to provide 4x papaya genome equivalents. High-density filters are being made for further characterization of this BAC library using plastid specific probes and selected low copy coffee genes. Our Arabica BAC library will be used for cloning genes controlling disease resistance and other economic traits so that they might be used for coffee improvement. In addition to applied research, this large insert genomic library will supplement existing coffee genomic resources for basic research such as understanding the basis for evolution of this allotetraploid genome from its two progenitor diploid genomes of C. canephora and C. eugenioides.