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United States Department of Agriculture

Agricultural Research Service

Title: NK-LYSIN: HIGH LEVEL OF LOCAL GENE EXPRESSION IN COCCIDIOSIS)

Author
item Lillehoj, Hyun
item Dalloul, Rami
item Miska, Kate
item Tuo, Wenbin
item Lee, Sung Hyen
item Han, Jae Yong
item Lillehoj, Erik

Submitted to: American Association of Immunologists Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 4/15/2006
Publication Date: 5/12/2006
Citation: Lillehoj, H.S., Hong, Y.H., Dalloul, R.A., Miska, K.B., Tuo, W., Lee, S., Han, J., Lillehoj, E.P. 2006. Nk-lysin: high level of local gene expression in coccidiosis.May 12-16, Boston, MA. P43.

Interpretive Summary:

Technical Abstract: NK-lysin is an anti-microbial and anti-tumor protein expressed by NK cells and T lymphocytes. A novel chicken gene encoding NK-lysin was cloned from the intestinal cDNA library which was produced from Eimeria-infected gut tissue. NK-lysin gene consists of 868 bp DNA with an open reading frame of 140 amino acids with a predicted molecular mass of 15.2 kDa. Comparison of its deduced amino acid sequence showed less than 20% identity to mammalian NK-lysins. High level of NK-lysin expression was detected in the intestinal intraepithelial lymphocytes (IELs), but lymphocytes from spleen, peripheral blood also showed significant levels of NK-lysin. In chickens orally infected with Eimeria maxima, NK-lysin transcript levels increased 3- to 4-fold in IEL with most expression occurring with CD4+ and CD8+ IELs. The kinetics of NK-lysin mRNA expression indicated that, whereas infection with E. acervulina induced maximum expression only at 7-8 days post-infection, E. maxima and E. tenella infections elicited biphasic responses at 3-4 and 7-8 days post-infection. Recombinant NK-lysin expressed in COS7 cells exhibited anti-tumor cell activity against LSCC-RP9, a tumor target for NK-cells. We conclude that chicken NK-lysin plays important roles during anti-microbial and anti-tumor defenses. (This project was supported, in part, by the National Research Initiative of the USDA CSREES, grant # 2004-35204-14798).

Last Modified: 8/24/2016
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