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Title: PROPAGATION AND MOLECULAR CHARACTERIZATION OF A SLOW-GROWING SUBGROUP A AVIAN LEUKOSIS VIRUS THAT WAS ORIGINALLY ISOLATED FROM COMMERCIAL MAREK'S DISEASE VACCINES

Author
item Fadly, Aly
item Silva, Robert
item TAYLOR, SCOTT - USDA-APHIS, AMES, IA

Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2006
Publication Date: 6/1/2006
Citation: Fadly, A.M., Silva, R.F., Taylor, S.P. 2006. Propagation and molecular characterization of a slow-growing subgroup A avian leukosis virus that was originally isolated from commercial Marek's disease vaccines [abstract]. American Veterinary Medical Association and American Association of Avian Pathologists Scientific Program. p. 72.

Interpretive Summary:

Technical Abstract: Attempts were made to propagate and obtain high titer of a slow-growing subgroup A avian leukosis virus (ALV-A) originally isolated from commercial Marek’s disease vaccines. Chicken-embryo fibroblasts (CEFs) from various chicken lines maintained at USDA-ARS-ADOL were used; also, various concentrations of chicken and calf sera were included in the culture media. Passing this slow-growing ALV-A in CEFs from a new ADOL line named RFS resulted in high titer virus stock, but only in cultures treated for 24 hours with 10% chicken serum. DNA sequence analysis indicates that this slow-growing ALV-A contains gp85 of exogenous subgroup A ALV and the LTR of endogenous subgroup E ALV. The finding that this isolate contains endogenous LTR may explain its slow growing characteristics in cell cultures and its low oncogenicity.