Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/27/2006
Publication Date: 3/31/2006
Citation: Tabatabai, L.B., Shinar, R., Shinar, J., Lager, K.M. 2006. A field-deployable fluorescence-based sensor excited by an organic light emitting device for PRRSV detection [abstract]. Stupka Memorial Symposium. Paper No. 14. p.13.
Technical Abstract: Porcine Reproductive and Respiratory syndrome Virus (PRRSV) infection costs the US swine industry $600 million annually in lost productivity. Although PRRSV can now be eliminated from individual farms, re-infection can not be prevented. PRRSV infection can be detected by virus isolation, serology, immunohistochemistry (IHC), polymerase chain reaction (PCR), and in situ hybridization (ISH). There are advantages and disadvantages to each of these tests. However, none of them are of a configuration that is easily deployable outside of a diagnostic laboratory. Here we describe a novel technology to develop an improved PRRSV diagnostic method that eventually will result in an inexpensive, compact, field deployable photoluminescence-based sensor for direct detection of PRRSV antigen, protein(s) or nucleic acid. Data are presented that show the various parameters for optimization for detection PRRSV by fluorescence detection methods, preparatory to deployment of the sensor device.