|Hare Jr, William|
Submitted to: American Society for Microbiology
Publication Type: Proceedings
Publication Acceptance Date: 3/21/2006
Publication Date: 4/21/2006
Citation: Solano Aguilar, G., Dawson, H.D., Shea-Donohue, T., Madden, K., Jones, Y.L., Beshah, E., Restrepo, M., Schoene, N.W., Hare Jr, W.R., Urban Jr, J.F. 2006. Biofiobacterium lactis enhances toll-like receptor (tlr) pathway gene expression locally in the colon and enhances glucose uptake in the small intestine of pigs infected with parasitic nematode ascaris sum. American Society for Microbiology Proceedings. 106:45A. Interpretive Summary:
Technical Abstract: The addition of probiotic bacteria to the diet is proposed to enhance healthy responses to allergic and infectious diseases in pediatric populations, but quantitative data is often lacking. An experimental model was developed to inoculate neonatal pigs from birth with B. lactis (Bb12), detect relative concentrations of Bb12 in the intestine, measure local tissue immune responses, and evaluate the response to an infectious challenge in the small intestine. Bb12 (3.5 x 1010 cfu), or a placebo-containing vehicle, was orally inoculated to pregnant sows during the last trimester of pregnancy and to their offspring from birth to weaning. Four experimental treatment groups were constructed as 1) Bb12-treated sows, Bb12-treated piglets (T/T), 2) Bb12-treated sows, placebo-treated (Control) piglets (T/C), 3) Placebo-treated sows, Bb12-treated piglets (C/T), and 4) Placebo-treated sows, placebo-treated piglets (C/C). Piglets from each treatment group were euthanized weekly after birth. Quantitative real-time PCR detection of the single copy Tuf gene was used to estimate the concentration of Bb12 in luminal samples and 46 selected immune biomarkers were measured in the intestinal mucosa and draining lymph nodes. A subset of piglets from the T/T and C/C groups were treated for six weeks after weaning and infected with A. suum for 21 days. Stripped sections of jejunal mucosae were mounted in Ussing chambers to determine changes in glucose absorption. Bb12 was detected in the highest concentrations in the proximal and distal colon in T/T > C/T> T/C> C/C piglets. Significant increases in TLR pathway gene expression for Tollip, IRAK4, TRAF6, TIRAP, and SARM were highly correlated (R2>0.56) with localized detection of Bb12. There was also a functional modification of the intestinal response to A. suum in the jejunum where the prototypical IL-4-dependent and Stat6-associated decrease in glucose absorption that accompanies fluid accumulation in the lumen was abrogated. The results suggest that a human-derived probiotic species can increase local innate immune responses in pigs and modulate allergic responses at other distal mucosal sites.